Oral hygiene agent containing hydrogen peroxide stabilized by colloidal silver

ABSTRACT

The present application is directed to oral hygiene agents, a process for their preparation, and the use of a hydrogen peroxide solution stabilized by a silver colloid in concentrations of from 0.1 to 10% by weight, preferably from 1 to 3% by weight, in oral hygiene agents.

This application is a 371 of PCT/EP92/01558 dated Jul. 9, 1992.

This invention is directed to oral hygiene agents, a process for their preparation, and a use.

Hitherto, hydrogen peroxide has been used as bleaching and cleaning agent. The use of hydrogen peroxide for decontaminating and deodorizing water, waste water and exhaust air instead of chlorine dioxide is familiar. For oral disinfection, a 3% hydrogen peroxide solution is suitable.

Use of hydrogen peroxide, particularly in the field of disinfection, gains increasing importance as this substance is considerably more environmentally compatible compared to chlorine-containing disinfectants used so far.

However, usability and storage stability of solutions containing hydrogen peroxide are limited in that hydrogen peroxide, particularly when exposed to heat, light, and in the presence of dust, traces of heavy metals and alkaline substances decomposes more or less rapidly to give water and oxygen. In disinfection or bleaching this is quite a desirable phenomenon since oxygen formed in decomposition has a disinfecting and bleaching effect. When storing such solutions, however, this is disadvantageous since solutions containing hydrogen peroxide decompose after a while and lose their disinfecting effect.

From EP-A-0 325 267, peroxide gels are known which contain from 35 to 95% by weight of polyols and, in addition, a stabilizer and hydrogen peroxide. As stabilizers are mentioned antioxidants or UV-absorbing materials such as butoxylated hydroxyanisole, hydroxytoluene, propyl gallate, or other substituted benzophenones. Although these are said to be toxicologically acceptable, they give rise to problems in part.

From AT-PS 349 640, a toothpaste free of polish is known which substantially consists of an oil-in-water emulsion. Hydrogen peroxide stabilization is effected using urea.

From EP-A-0 288 420, an aqueous hydrogen peroxide gel is known which is gelled with polyoxyethylene/polyoxyproplyene block copolymers and contains polyethylene glycol for moisturizing. The substances utilized therein, in particular Pluronics, are not quite toxicologically safe.

Therefore, it was the technical problem of the invention to provide a stabilized hydrogen peroxide for oral hygiene agents which, on the one hand, permits production of usable and storage-stable oral hygiene agents and, on the other hand, has the familiar advantageous disinfecting effect of hydrogen peroxide.

This technical problem is solved by an oral hygiene agent containing, in addition to common ingredients, a silver colloid-stabilized hydrogen peroxide solution in concentrations of from 0.1 to 10% by weight, preferably from 1 to 3% by weight. Such a stabilized hydrogen peroxide solution is available, for instance, from the company Hungerbach-Chemotechnik GmbH under the designation HUWA-SAN. This solution is stabilized by a silver colloid.

The oral hygiene agent of the invention is prepared by mixing common ingredients with said stabilized hydrogen peroxide solution.

Furthermore, the technical problem of the invention is solved by using a silver colloid-stabilized hydrogen peroxide solution for oral hygiene in concentrations of from 0.1 to 10% by weight, preferably from 1 to 3% by weight.

The silver colloid-stabilized hydrogen peroxide solution may be utilized in a variety of oral hygiene agents including toothpaste, toothpaste for sensitive teeth generally containing soluble potassium and strontium salts or hydroxylapatite, mouth wash, dental powder, and chewing gum for dental care. Furthermore, utilization in oral hygiene agents for artificial teeth such as adhesives, adhesive foams and cleaning agents for artificial teeth is possible. The stabilized hydrogen peroxide solution may also be used as a concentrate or a ready-for-use solution to fill up oral douches.

Despite stabilization, outstanding disinfecting effect is obtained by using the silver colloid-stabilized hydrogen peroxide solution. Thus, the disinfecting effect of a stabilized H₂ O₂ solution (HUWA-SAN) was investigated. Here, it was determined that effective disinfection in clinical water-bearing systems may be achieved by using stabilized hydrogen peroxide.

Furthermore, a disinfection test was carried out using various germs and various concentrations of stabilized hydrogen peroxide (HUWA-SAN). As can be seen from table 1, no growth can be detected in any strain with concentrations of up to 0.0075%. On the basis of these results, the stabilized hydrogen peroxide solution has significant bacteriostatic and fungistatic activity. The minimum concentration for inhibition is about 5 ppm. On the average, a value for all germs of 30 ppm may be expected.

                                      TABLE 1                                      __________________________________________________________________________     Concentration-dependent germ inhibition by                                     stabilized hydrogen peroxide solution (HUWA-SAN)                                       Employed germ                                                                          Testing concentration in %                                     Tested germ                                                                            content/ml                                                                             10                                                                               1 0.1                                                                              0.05                                                                              0.01                                                                              0.0075                                                                             0.005                                                                             0.002                                                                             0.001                                                                             0.0005                                __________________________________________________________________________     Escherichia                                                                            53 · 10.sup.6                                                                 --                                                                               --                                                                               --                                                                               -- -- --  -- -- -- (+)                                   coli                                                                           Klebsiella                                                                             6.1 · 10.sup.6                                                                --                                                                               --                                                                               --                                                                               -- -- --  -- -- -- +                                     pneumoniae                                                                     Staphylo-                                                                              50 · 10.sup.6                                                                 --                                                                               --                                                                               --                                                                               -- -- --  -- -- --                                       coccus                                                                         Pseudomonas                                                                            70 · 10.sup.6                                                                 --                                                                               --                                                                               --                                                                               -- -- --  -- +  ++ ++                                    aeruginosa                                                                     Streptococcus                                                                          36 · 10.sup.6                                                                 --                                                                               --                                                                               --                                                                               -- -- --  -- -- -- (+)                                   faecalis                                                                       Proteus 24 · 10.sup.6                                                                 --                                                                               --                                                                               --                                                                               -- -- --  -- -- -- --                                    mirabilis                                                                      Mycobacterium                                                                          1.5 · 10.sup.6                                                                --                                                                               --                                                                               --                                                                               -- -- --  (+)                                                                               +  +  +                                     tuberculosis                                                                   Clostridium                                                                            2.1 · 10.sup.6                                                                --                                                                               --                                                                               --                                                                               -- -- --  -- +  ++ ++                                    sporogenes                                                                     Candida 3.2 · 10.sup.6                                                                --                                                                               --                                                                               --                                                                               -- -- --  (+)                                                                               ++ ++ ++                                    albicans                                                                       __________________________________________________________________________      Key to symbols:                                                                -- = no growth                                                                 (+) = faint growth                                                             + = significant growth                                                         ++ = massive growth                                                            BLW = Control value with sterile water                                   

In another study, the disinfecting effect in oral hygiene agents was determined using a 2% stabilized hydrogen peroxide solution (HUWA-SAN). Here, the germs Pseudomonas aeruginosa, Candida albicans and Stapihylococcus aureus were used. For testing, plastic plates of prosthetic plastic having a size of 2.5 cm×2.5 cm×0.3 cm were produced. The test specimen were microbially contaminated using following contamination solution: 1

    ______________________________________                                         10 ml    of 0.9% sterile sodium chloride solution with the                              centrifugate of a 200 ml breed of one of the cul-                              tures,                                                                83 ml    of 5% sterile mucin solution,                                          2 ml    of 5% sterile calcium chloride solution.                              ______________________________________                                    

The test specimen were placed into the contamination solution for 30 seconds. They were taken out using a pair of forceps and subsequently laid onto a fine-meshed grid. There, first drying was effected for 15 minutes at room temperature. The contaminated test specimen were foamed from all sides using adhesive foam which was spread using a sterile glass spatula. After the periods of exposure had elapsed, the test specimen were placed onto a sterile cloth of cotton using a pair of forceps and were freed from adhering foam.

The number of germs was determined by shaking out into the following extraction solution:

Substances in 100 ml of CSL

3% of Tween 80

3% of saponine

0.1% of histidine

0.1% of cysteine.

After 2 minutes of shaking, the germ count was performed. After an exposure period of 1 minute, there was found germ reduction for

    ______________________________________                                         Pseudomonas aeruginosa to 2%                                                   Candida albicans       to 0.06%                                                Staphylococcus aureus  to 0%.                                                  ______________________________________                                    

From these results, very good disinfecting activity of the stabilized hydrogen peroxide solutions can be derived. Further results can be seen from table 2.

                  TABLE 2                                                          ______________________________________                                         Investigations concerning inhibiting effect in a foam                          formulation, 2% hydrogen peroxide solution (HUWA-SAN).                                Period of exposure (minutes)                                            Test specimen                                                                           0.5       1         1.5     3                                         ______________________________________                                         Test germ: Pseudomonas aeruginosa, 1 · 10.sup.9                       colony-forming units per ml                                                    I        2.5 · 10.sup.5                                                                  4.5 · 10.sup.4                                                                  1.5 · 10.sup.4                                                                0                                         II        3 · 10.sup.5                                                                   5 · 10.sup.4                                                                      2 · 10.sup.4                                                                0                                         III       3 · 10.sup.5                                                                   4 · 10.sup.4                                                                    1.5 · 10.sup.4                                                                0                                         Control  2.9 · 10.sup.6                                                                  2.2 · 10.sup.6                                                                  2.2 · 10.sup.6                                                                2.1 · 10.sup.6                   Reduction to                                                                            10%       2%        8%      0                                         about                                                                          Test germ: Candida albicans, 5.5 · 10.sup.8                           colony-forming units per ml                                                    I          3 · 10.sup.3                                                                  1 · 10.sup.3                                                                    6 · 10.sup.2                                                                   1 · 10.sup.1                    II       3.5 · 10.sup.3                                                                  2 · 10.sup.3                                                                    8 · 10.sup.2                                                                  0                                         II         5 · 10.sup.3                                                                  9 · 10.sup.2                                                                    8.5 · 10.sup.2                                                                0                                         Control  1.9 · 10.sup.6                                                                  2 · 10.sup.6                                                                    7 · 10.sup.5                                                                  1 · 10.sup.5                     Reduction to                                                                            0.2%      0.06%     0.1%    0.003%                                    about                                                                          Test germ: Staphylococcus aureus, 2 · 10.sup.9                        colony-forming units per ml                                                    I          4 · 10.sup.1                                                                  0         3 · 10.sup.1                                                                  0                                         II         2 · 10.sup.1                                                                  0         0       0                                         III      3.5 · 10.sup.1                                                                  0         1 · 10.sup.1                                                                  0                                         Control  3.5 · 10.sup.6                                                                  3.7 · 10.sup.6                                                                  3.7 · 10.sup.6                                                                3.3 · 10.sup.6                   Reduction to                                                                            0.0009%   0         0.0003% 0                                         about                                                                          ______________________________________                                    

The further examples demonstrate preferred embodiments of the invention:

EXAMPLE 1 Mouthwash Formulation

In convential manner, there are mixed

7 wt.-% of ethanol,

17 wt.-% of sorbitol solution

2 wt.-% of stabilized H₂ O₂ solution (HUWA-SAN)

1 wt.-% of common additives

73 wt.-% of water.

EXAMPLE 2 Adhesive Foam

There are mixed

1 wt.-% of Polyisosorbat 20

0.45 wt.-% of mint oils

5 wt.-% of alcohol

2.20 wt.-% of sodium benzoate

3 wt.-% of sodium lauryl sulfate

5 wt.-% of propane/butane

2 wt.-% of stabilized hydrogen peroxide solution (HUWA-SAN)

1 wt.-% of common additives

80.35 wt.-% of water.

EXAMPLE 3 Toothpaste A

12 wt.-% of diatomaceous earth

1.6 wt.-% of hydroxyethylcellulose

12 wt.-% of glycerol

2 wt.-% of silica

12 wt.-% of sorbitol/xylitol solution

10 wt.-% of strontium chloride

1 wt-.% of peppermint oil

2 wt.-% of stabilized hydrogen peroxide solution (HUWA-SAN)

5 wt.-% of common additives

42.4 wt.-% of water.

EXAMPLE 4 Toothpaste B

5 wt.-% of potassium nitrate

1 wt.-% of sodium monofluorophosphate

36 wt.-% of dicalcium phosphate

12 wt.-% of sorbitol/xylitol solution

12 wt.-% of glycerol

1 wt-.% of peppermint oil

2 wt.-% of stabilized hydrogen peroxide solution (HUWA-SAN)

5 wt.-% of common additives

38 wt.-% of water. 

We claim:
 1. In a hydrogen-peroxide-containing oral hygiene-composition, the improvement wherein the hydrogen peroxide is silver-colloid-stabilized hydrogen peroxide present at a concentration of 0.1 to 10% by weight.
 2. The oral hygiene composition according to claim 1, characterized in that the concentration of said stabilized hydrogen peroxide is from 1 to 3% by weight.
 3. The composition according to claim 1, characterized in that the oral hygiene composition is toothpaste, mouthwash, dental powder, dental adhesive, cleaning agent for artificial teeth, chewing gum for dental care, or an oral douche.
 4. The composition according to claim 2, characterized in that the oral hygiene composition is toothpaste, mouthwash, dental powder, dental adhesive, cleaning agent for artificial teeth, chewing gum for dental care, or an oral douche.
 5. A method of using a silver-colloid-stabilized hydrogen peroxide solution comprising adding the solution to an oral hygiene composition, at a concentration of from 0.1 to 10% by weight.
 6. The method according to claim 5, characterized in that the stabilized hydrogen peroxide solution is added at a concentration of from 1 to 3% by weight.
 7. The method according to claim 5, characterized in that the oral hygiene composition is toothpaste, mouthwash, dental powder, dental adhesive, cleaning agent for artificial teeth, chewing gum for dental care, or an oral douche.
 8. The method according to claim 6, characterized in that the oral hygiene composition is toothpaste, mouthwash, dental powder, adhesive, cleaning agent for artificial teeth, chewing gum for dental care, or an oral douche.
 9. The method of claim 5, wherein the composition is toothpaste comprising a soluble potassium salt, a soluble strontium salt, or hydroxyapatite.
 10. The method of claim 6, wherein the composition is toothpaste comprising a soluble potassium salt, a soluble strontium salt, or hydroxyapatite. 